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LiftOn is a homology-based lift-over tool using both DNA-DNA alignments (from Liftoff, credits to Dr. Alaina Shumate) and protein-DNA alignments (from miniprot, credits to Dr. Heng Li) to accurately map annotations between genome assemblies of the same or different species. LiftOn employs a two-step protein maximization algorithm to improve the annotation of protein-coding genes in the T2T-CHM13.

Splam is a splice site predictor utilizing a deep residual convolutional neural network for fast and accurate evaluation of splice junctions solely based on 400nt DNA sequences around donor and acceptor sites.